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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important role in selling mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already described. Two more splice variants have been reported but detailed nucleotide sequence info is not still obtainable.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified from the antibody.
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This tutorial outlines more achievable optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide no more bind to the epitope within the focus on protein. By evaluating the staining within the blocked antibody compared to the antibody by yourself, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs for the really conserved cyclin household, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates at the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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